Journal: Poultry science

Article Title: The effects of arginine and branched-chain amino acid supplementation to reduced-protein diet on intestinal health, cecal short-chain fatty acid profiles, and immune response in broiler chickens challenged with Eimeria spp.

doi: 10.1016/j.psj.2023.102773

Figure Lengend Snippet: Figure 3. Effects of reduced-protein diets supplemented with argi- nine or branched chain amino acids on cecal tonsil CD25+CD4+ cell (Tregs) percentage in broiler chickens challenged with Eimeria spp. Tregs was presented as percentage of CD4+ cells on d 21 and 28. PC, positive control with 20.0% crude protein; NC, negative control with 17.5% crude protein; ARG and BCAA, negative control supplemented with arginine or BCAA. The error bars represent the SEM values. (A), Bars without a common letter differ significantly. P-values: P-challenge < 0.001, P-diet = 0.646, P-interaction = 0.010. (B), The asterisk (*) denoted significant difference. P-values: P-challenge < 0.001, P- diet = 0.507, P-interaction = 0.089.

Article Snippet: For CD4+CD25+ cell analysis, the cells were incubated with 1 mg/mL mouse antichicken CD25 antibody (Shanmugasundaram and Selvaraj, 2011) and unlabeled mouse IgG at 1:100 dilution in a 96-well plate for 50 min at 4°C, followed by incubation with a 1:250 dilution of FITC-conjugated mouse antichicken CD4 (Southern Biotech) for 20 min at 4°C.

Techniques: Positive Control, Negative Control

Journal: Veterinary Research

Article Title: The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens

doi: 10.1186/s13567-023-01257-8

Figure Lengend Snippet: Primary antibodies used for multiparametric staining for flow cytometry

Article Snippet: Chicken CD25 , AV142 , pure , Bio-Rad AbD serotec , 1 μg/mL.

Techniques: Staining

Journal: Veterinary Research

Article Title: The role of PB1-F2 in adaptation of high pathogenicity avian influenza virus H7N7 in chickens

doi: 10.1186/s13567-023-01257-8

Figure Lengend Snippet: PB1-F2 marginally affects frequencies of leukocyte subsets. After ex vivo isolation leukocytes were stained for multiparametric flow cytometry. Single, live T cells (CD3 + , A – F ) or single, live antigen presenting cells (APC, CD4−, MHC-II+ , G – H ) were phenotyped. A Frequencies of γδ T cells, B Activation (CD8α +) of γδ T cells, C Frequencies of Effector T cells (CD8 +) from αβ T cell fraction, D CD4 + CD8 + αβ T cells, E T helper cells (CD4 +) from αβ T cell fraction, F percentage of activated (CD25 +) T helper cells, G Monocyte (KUL-01 +) fraction of APC, H Fraction of B cells (Bu-1 +) of APC. Statistical analysis was performed as two-way ANOVA with Tukey’s multiple comparisons test. Data is shown as mean (SD), control (white), wt (blue), ΔF2 (red).

Article Snippet: Chicken CD25 , AV142 , pure , Bio-Rad AbD serotec , 1 μg/mL.

Techniques: Ex Vivo, Isolation, Staining, Flow Cytometry, Activation Assay, Control

Journal: The Journal of biological chemistry

Article Title: The epithelial sodium-hydrogen antiporter Na+/H+ exchanger 3 accumulates and is functional in recycling endosomes.

doi: 10.1074/jbc.273.4.2035

Figure Lengend Snippet: FIG. 4. Comparison of the distribu- tion of NHE-3HA with markers of the Golgi cisternae, TGN, and lysosomes. A–C, NHE3-HA-transfected cells were fixed, permeabilized, and stained using monoclonal anti-HA antibodies, as in Fig. 2. D, the cells in A were also immuno- stained with polyclonal antibodies to a-mannosidase II, a marker of medial and trans-Golgi cisternae (25). E, the cells in B were stably transfected with a TGN38- CD25 chimera, as described under “Mate- rials and Methods.” This chimeric marker of the TGN (26) was labeled using anti- CD25 antibodies. F, prior to fixation, the cells in C were allowed to internalize fix- able fluoresceinated dextran for 12 h. Af- ter a 4-h chase, to clear the dextran from the endosomal compartment, the cells were fixed and immunostained. Insets near the top right corners of A–E are mag- nifications of the areas demarcated by dotted lines. Confocal images are repre- sentative of at least three experiments.

Article Snippet: FITC-labeled anti-T-cell antigen (CD25) Ab was from Serotec.

Techniques: Comparison, Transfection, Staining, Marker, Stable Transfection, Labeling

Journal: Toxins

Article Title: Exposure to Subclinical Doses of Fumonisins, Deoxynivalenol, and Zearalenone Affects Immune Response, Amino Acid Digestibility, and Intestinal Morphology in Broiler Chickens

doi: 10.3390/toxins17010016

Figure Lengend Snippet: Effect of combined doses of mycotoxins on cecal tonsils CD4+, CD8+ ratio, and Tregs percentage. Flow cytometry was used to analyze the percentages of CD4+, CD8+ T, and CD4+CD25+ T regulatory cells in the cecal tonsils. Means within a row with no common superscript differ significantly ( p < 0.05) ( n = 6).

Article Snippet: For the T-regulatory cell (Tregs) percentages, cells (1 × 10 6 ) were incubated with 10 μg/mL of phycoerythrin-conjugated mouse anti-chicken CD25 antibody [ ], 1:250 dilution of FITC-d- conjugated mouse anti-chicken CD4+ (Southern Biotechnology Associates, Birmingham, AL, USA), and a 1:200 dilution of unlabeled mouse IgG for 45 min.

Techniques: Flow Cytometry, Control

Journal: Toxins

Article Title: Exposure to Subclinical Doses of Fumonisins, Deoxynivalenol, and Zearalenone Affects Immune Response, Amino Acid Digestibility, and Intestinal Morphology in Broiler Chickens

doi: 10.3390/toxins17010016

Figure Lengend Snippet: Effect of combined doses of mycotoxins on spleen CD4+, CD8+, and Tregs percentage. CD4+ and CD8+ T cells and CD4 + CD25 + T regulatory cell percentages in splenocytes were analyzed by flow cytometry. Means within a row with no common superscript differ significantly ( p < 0.05) ( n = 6).

Article Snippet: For the T-regulatory cell (Tregs) percentages, cells (1 × 10 6 ) were incubated with 10 μg/mL of phycoerythrin-conjugated mouse anti-chicken CD25 antibody [ ], 1:250 dilution of FITC-d- conjugated mouse anti-chicken CD4+ (Southern Biotechnology Associates, Birmingham, AL, USA), and a 1:200 dilution of unlabeled mouse IgG for 45 min.

Techniques: Flow Cytometry, Control